MESSAGE AMPLIFICATION PHENOTYPING (MAPPING) - PRINCIPLES, PRACTICE AND POTENTIAL

被引:24
作者
LARRICK, JW
机构
[1] Palo Alto Institute for Molecular Medicine, Stanford, CA 94309-8102
关键词
D O I
10.1016/0167-7799(92)90202-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Message amplification phenotyping (MAPPing)* is a sensitive polymerase chain reaction (PCR)-based technique for the rapid determination of the mRNA phenotype of small numbers of cells. Isolated mRNA is reverse-transcribed (RT) into DNA, and then the DNA fragments corresponding to proteins or genes of interest are specifically amplified by PCR. The technique, also called RT-PCR, has wide application in biology and medicine, and comparison with bioassays demonstrates that MAPPing saves significant time and material. The technique should be applicable to analyse mRNAs of virtually any tissue or cell type.
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页码:146 / 152
页数:7
相关论文
共 33 条
  • [1] METHOD FOR DETECTION OF SPECIFIC RNAS IN AGAROSE GELS BY TRANSFER TO DIAZOBENZYLOXYMETHYL-PAPER AND HYBRIDIZATION WITH DNA PROBES
    ALWINE, JC
    KEMP, DJ
    STARK, GR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) : 5350 - 5354
  • [2] IDENTIFICATION OF ATTENUATING MUTATIONS ON THE REOVIRUS TYPE-3 S1 DOUBLE-STRANDED-RNA SEGMENT WITH A RAPID SEQUENCING TECHNIQUE
    BASSELDUBY, R
    SPRIGGS, DR
    TYLER, KL
    FIELDS, BN
    [J]. JOURNAL OF VIROLOGY, 1986, 60 (01) : 64 - 67
  • [3] DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS CERVICAL INFECTION BY DETECTION OF AMPLIFIED DNA WITH AN ENZYME-IMMUNOASSAY
    BOBO, L
    COUTLEE, F
    YOLKEN, RH
    QUINN, T
    VISCIDI, RP
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (09) : 1968 - 1973
  • [4] BRENNER CA, 1989, BIOTECHNIQUES, V7, P1096
  • [5] GENES FOR EXTRACELLULAR MATRIX-DEGRADING METALLOPROTEINASES AND THEIR INHIBITOR, TIMP, ARE EXPRESSED DURING EARLY MAMMALIAN DEVELOPMENT
    BRENNER, CA
    ADLER, RR
    RAPPOLEE, DA
    PEDERSEN, RA
    WERB, Z
    [J]. GENES & DEVELOPMENT, 1989, 3 (06) : 848 - 859
  • [6] BUELL GN, 1978, J BIOL CHEM, V253, P2471
  • [7] CHAPMAN AB, 1987, MOL CELL BIOL, V3, P1421
  • [8] QUANTITATIVE ESTIMATION OF MINOR MESSENGER-RNAS BY CDNA-POLYMERASE CHAIN-REACTION - APPLICATION TO DYSTROPHIN MESSENGER-RNA IN CULTURED MYOGENIC AND BRAIN-CELLS
    CHELLY, J
    MONTARRAS, D
    PINSET, C
    BERWALDNETTER, Y
    KAPLAN, JC
    KAHN, A
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 187 (03): : 691 - 698
  • [9] CHIRGWIN JM, 1979, BIOCHEMISTRY-US, V15, P5294
  • [10] IMMUNODETECTION OF DNA WITH BIOTINYLATED RNA PROBES - A STUDY OF REACTIVITY OF A MONOCLONAL-ANTIBODY TO DNA RNA HYBRIDS
    COUTLEE, F
    BOBO, L
    MAYUR, K
    YOLKEN, RH
    VISCIDI, RP
    [J]. ANALYTICAL BIOCHEMISTRY, 1989, 181 (01) : 96 - 105