ODOR DEPRIVATION LEADS TO REDUCED NEUROGENESIS AND REDUCED NEURONAL SURVIVAL IN THE OLFACTORY-BULB OF THE ADULT-MOUSE

被引:135
作者
COROTTO, FS
HENEGAR, JR
MARUNIAK, JA
机构
[1] UNIV MISSOURI, DIV BIOL SCI, COLUMBIA, MO 65211 USA
[2] UNIV MISSOURI, DEPT MED, COLUMBIA, MO 65211 USA
关键词
D O I
10.1016/0306-4522(94)90397-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neurogenesis persists in the olfactory bulbs of adult mice, with new cells being generated in the proliferative subependymal layer. Our previous work has shown that unilateral odor deprivation through naris closure leads to a net loss of granule neurons in the ipsilateral (odor-deprived) olfactory bulb, while not affecting the contralateral bulb. Here we used several experimental approaches to determine if this loss of neurons results from reduced neurogenesis, reduced neuronal survival, or both. First, bromodeoxyuridine immunohistochemistry was used to determine the number of S-phase cells in the subependymal layer eight weeks after naris closure. Proliferation was reduced within and just caudal to the odor-deprived bulb compared to the open-side (control) bulb. Second, counts of pyknotic nuclei four weeks after naris closure were used to document a higher rate of cell death on the deprived side. Third, H-3-thymidine autoradiography was used to assess differences in granule cell survival on the two sides. Granule cell precursors were labeled by a single injection of H-3-thymidine eight weeks after naris closure, and the number of surviving labeled granule cells assessed four and 16 weeks later. Granule cell survival was significantly reduced within the odor-deprived bulbs. These data indicate that the loss of granule cells which follows odor deprivation is caused, at least in part, by reduced neurogenesis and reduced survival of these adult-generated neurons.
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页码:739 / 744
页数:6
相关论文
共 32 条
[1]   ESTIMATION OF NUCLEAR POPULATION FROM MICROTOME SECTIONS [J].
ABERCROMBIE, M .
ANATOMICAL RECORD, 1946, 94 (02) :239-247
[2]   AUTORADIOGRAPHIC AND HISTOLOGICAL STUDIES OF POSTNATAL NEUROGENESIS .4. CELL PROLIFERATION AND MIGRATION IN ANTERIOR FOREBRAIN, WITH SPECIAL REFERENCE TO PERSISTING NEUROGENESIS IN OLFACTORY BULB [J].
ALTMAN, J .
JOURNAL OF COMPARATIVE NEUROLOGY, 1969, 137 (04) :433-&
[3]  
BAYER SA, 1983, EXP BRAIN RES, V50, P329
[4]  
BENSON TE, 1984, J NEUROSCI, V4, P638
[5]   UNILATERAL ODOR DEPRIVATION - TIME COURSE OF CHANGES IN LAMINAR VOLUME [J].
BRUNJES, PC .
BRAIN RESEARCH BULLETIN, 1985, 14 (03) :233-237
[6]   UNILATERAL OLFACTORY DEPRIVATION - EFFECTS ON SUCCINATE-DEHYDROGENASE HISTOCHEMISTRY AND [H-3] LEUCINE INCORPORATION IN THE OLFACTORY MUCOSA [J].
BRUNJES, PC ;
CAGGIANO, AO ;
KOROL, DL ;
STEWART, JS .
DEVELOPMENTAL BRAIN RESEARCH, 1991, 62 (02) :239-244
[7]  
Corotto Frank, 1992, Chemical Senses, V17, P606
[8]   NEUROGENESIS PERSISTS IN THE SUBEPENDYMAL LAYER OF THE ADULT-MOUSE BRAIN [J].
COROTTO, FS ;
HENEGAR, JA ;
MARUNIAK, JA .
NEUROSCIENCE LETTERS, 1993, 149 (02) :111-114
[9]   UNILATERAL ODOR DEPRIVATION - EFFECTS ON THE DEVELOPMENT OF STAINING FOR OLFACTORY-BULB SUCCINATE-DEHYDROGENASE [J].
CULLINAN, WE ;
BRUNJES, PC .
DEVELOPMENTAL BRAIN RESEARCH, 1987, 35 (01) :35-42
[10]  
DAVIS MR, 1983, J COMP NEUROL, V221, P444, DOI 10.1002/cne.902210407