EFFECTS OF FIXED-CHARGE DISTRIBUTION AND PH ON THE ELECTROPHORETIC MOBILITY OF BIOLOGICAL CELLS

被引:19
作者
HSU, JP [1 ]
HSU, WC [1 ]
CHANG, YI [1 ]
机构
[1] TUNGHAI UNIV,DEPT CHEM ENGN,TAICHUNG,TAIWAN
关键词
ELECTROPHORETIC MOBILITY; CELLS; FIXED CHARGE; DONNAN POTENTIAL;
D O I
10.1007/BF00655495
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The electrophoretic mobility of biological cells is investigated theoretically. In particular, the effects of the distribution of the charges in the surface layer and the pH of bulk liquid phase on the mobility of cells are examined. The former includes the fixed charges due to the dissociation of the functional groups and the charges due to the penetrated electrolyte ions. The present analysis extends previous results in that the fixed charges are distributed nonuniformly across the surface layer of a cell. It is found that the distribution of the fixed charges in the surface layer has a significant effect on its electrophoretic mobility. Thus, assuming that the fixed charges are homogeneously distributed in the surface layer of a cell may lead to a significant deviation.
引用
收藏
页码:251 / 260
页数:10
相关论文
共 25 条
[2]  
CHAN DYC, 1975, J CHEM SOC F1, V70, P1046
[4]   DIVALENT-CATIONS CAN INCREASE CELL-SUBSTRATE REPULSION AT SMALL DISTANCES [J].
CHANG, YI .
COLLOIDS AND SURFACES, 1989, 41 (3-4) :245-254
[5]   THE EFFECT OF MULTIVALENT CATIONS ON ADHESION TIME FOR CELLULAR ADHESION TO SOLID-SURFACES [J].
CHANG, YI ;
HSU, JP .
JOURNAL OF THEORETICAL BIOLOGY, 1990, 147 (04) :509-516
[6]   ELECTROPHORETICAL STUDY OF CELL-SURFACE PROPERTIES - INFLUENCE OF THE SURFACE-COAT ON THE ELECTRIC-POTENTIAL DISTRIBUTION AND ON GENERAL ELECTROKINETIC PROPERTIES OF ANIMAL-CELLS [J].
DONATH, E ;
PASTUSHENKO, V .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1979, 6 (04) :543-554
[7]   IONIZABLE SURFACE GROUP MODELS OF AQUEOUS INTERFACES [J].
HEALY, TW ;
WHITE, LR .
ADVANCES IN COLLOID AND INTERFACE SCIENCE, 1978, 9 (04) :303-345
[8]  
HSU JP, 1993, J COLLOID INTERF SCI, V155, P1
[9]  
HUNTER RJ, 1989, FDN COLLOID SCI, V1, P557
[10]   THEORY OF THE ELECTROKINETIC BEHAVIOR OF HUMAN-ERYTHROCYTES [J].
LEVINE, S ;
LEVINE, M ;
SHARP, KA ;
BROOKS, DE .
BIOPHYSICAL JOURNAL, 1983, 42 (02) :127-135