EFFECT OF ALPHA-TOXIN AND CAPSULAR EXOPOLYSACCHARIDE ON THE ADHERENCE OF STAPHYLOCOCCUS-AUREUS TO CULTURED TEAT, DUCTAL AND SECRETORY MAMMARY EPITHELIAL-CELLS

被引：20

作者：

CIFRIAN, E

GUIDRY, AJ

OBRIEN, CN

MARQUARDT, WW

机构：

[1] UNIV MARYLAND,DEPT ANIM SCI,COLLEGE PK,MD 20742

[2] UNIV MARYLAND,VIRGINIA MARYLAND REG COLL VET MED,COLLEGE PK,MD 20742

来源：

RESEARCH IN VETERINARY SCIENCE | 1995年 / 58卷 / 01期

关键词：

D O I：

10.1016/0034-5288(95)90083-7

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Cultures of teat, ductal and secretory epithelial cells were used to study the role of alpha-toxin and the capsular exopolysaccharide on the adherence of Staphylococcus aureus to mammary epithelium. The adherence of S aureus to the cells and their susceptibility to damage by alpha-toxin increased from teat to ductal to secretory cells. Alpha-toxin increased the susceptibility of epithelial cell monolayers to adherence by S aureus, and the extent of the adherence increased with the time of exposure to alpha-toxin. The exopolysaccharide capsule deterred the adherence of S aureus to mammary epithelial cells and to collagen. Organisms with a rigid capsule adhered to a smaller extent than those with a flaccid capsule. Both encapsulated and unencapsulated S aureus adhered more readily to collagen than to either healthy monolayers of epithelial cells or monolayers of cells damaged by alpha-toxin.

引用

页码：20 / 25

页数：6

共 46 条

[1] FACTORS INFLUENCING THE DEGREE OF INVITRO BACTERIAL ADHESION TO OVINE MAMMARY-GLAND EPITHELIAL-CELLS [J].