CELL-CYCLE KINETICS IN FETAL-RAT CEREBRAL-CORTEX - EFFECTS OF PRENATAL TREATMENT WITH ETHANOL ASSESSED BY A CUMULATIVE LABELING TECHNIQUE WITH PLOW CYTOMETRY

被引:54
作者
MILLER, MW
KUHN, PE
机构
[1] UNIV IOWA,COLL MED,DEPT PHARMACOL,IOWA CITY,IA 52242
[2] VET AFFAIRS MED CTR,RES SERV,IOWA CITY,IA 52242
[3] RUTGERS STATE UNIV,DEPT BIOL,PROGRAM CELL & DEV BIOL,PISCATAWAY,NJ
[4] UNIV IOWA,COLL MED,DEPT PSYCHIAT,IOWA CITY,IA 52242
关键词
ALCOHOL; CELL PROLIFERATION; CYTOKINETICS; FETAL ALCOHOL SYNDROME; VENTRICULAR ZONE;
D O I
10.1111/j.1530-0277.1995.tb01497.x
中图分类号
R194 [卫生标准、卫生检查、医药管理];
学科分类号
摘要
The effects of ethanol on the cell cycle kinetics of cortical precursor cells during the period of cortical neuronogenesis [between gestational day (G) 12 and G21] was systematically examined. Samples of dissociated cortical cells were harvested from the cerebral cortices of 13-, 15-, 17-, 19-, and 21-day-old fetuses. The fetuses were obtained from pregnant rats: (a) fed a liquid diet containing 6.7% (v/v) ethanol (Et) ad libitum, (b) pair-fed an isocaloric liquid control diet (Ct), or (c) fed chow and water (Ch) ad libitum. Before harvesting the cells, the fetuses were administered a series of 1-5 injections of bromodeoxyuridine (BrdU). The proportion of cells that incorporated the BrdU was assessed. Using these raw data, the S-phase length (T-s, total cell cycle length (T-c, and the growth fraction (GF; the fraction of the total population that was actively cycling) were determined with a cumulative labeling procedure. The T-s was similar to 8-9 hr, regardless of either the date of the injection or the dietary treatment of the dam. On the other hand, the T-c for the Ct- and Ch-treated rats increased over the gestational period. That is, the Tc was shortest on G13 and longest on G21. The T-c for Et-treated rats, however, did not change between G13 and G21. For the Ch- and Ct-treated groups, the GF decreased >15-fold between G13 and G21. The decline (5-fold) for the Et-treated group over the same period, however, was not as great as it was for the Ct-treated fetuses. Thus, by G17 (and thereafter), the GF for Et-treated fetuses was significantly greater than it was for the Ct-treated group. Ethanol treatment has opposite effects on the two cortical germinal zones; it stimulates the proliferation of subventricular cells, whereas it inhibits the proliferation of ventricular cells). Thus, the ethanol-induced changes in the T-c and the GF reflect the combined effects of ethanol on the two cortical proliferative zones.
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页码:233 / 237
页数:5
相关论文
共 42 条
[1]   HYPOPLOIDY AND HYPERPLASIA IN THE DEVELOPING BRAIN EXPOSED TO ALCOHOL INUTERO [J].
ALVAREZ, MR ;
STONE, DJ .
TERATOLOGY, 1988, 37 (03) :233-238
[2]   EFFECT OF ETHANOL CHRONICALLY ADMINISTERED TO PREWEANLING RATS ON CEREBELLAR DEVELOPMENT - MORPHOLOGICAL-STUDY [J].
BAUERMOFFETT, C ;
ALTMAN, J .
BRAIN RESEARCH, 1977, 119 (02) :249-268
[3]  
Bayer S. A., 1991, NEOCORTICAL DEV
[4]   ALCOHOL-INDUCED NEURONAL LOSS IN DEVELOPING RATS - INCREASED BRAIN-DAMAGE WITH BINGE EXPOSURE [J].
BONTHIUS, DJ ;
WEST, JR .
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 1990, 14 (01) :107-118
[5]   EFFECTS OF ETHANOL ON POSTNATAL CELL ACQUISITION IN THE RAT CEREBELLUM [J].
BORGES, S ;
LEWIS, PD .
BRAIN RESEARCH, 1983, 271 (02) :388-391
[6]   NEUROGENESIS IN VISUAL SYSTEM OF RAT - AUTORADIOGRAPHIC INVESTIGATION [J].
BRUCKNER, G ;
MARES, V ;
BIESOLD, D .
JOURNAL OF COMPARATIVE NEUROLOGY, 1976, 166 (02) :245-+
[7]   IMMUNOTOXICITY OF ALCOHOL IN YOUNG AND OLD MICE .2. IMPAIRED T-CELL PROLIFERATION AND T-CELL-DEPENDENT ANTIBODY-RESPONSES OF YOUNG AND OLD MICE FED ETHANOL-CONTAINING LIQUID DIET [J].
CHANG, MP ;
NORMAN, DC .
MECHANISMS OF AGEING AND DEVELOPMENT, 1991, 57 (02) :175-186
[8]   SELECTIVE EFFECTS OF FETAL ALCOHOL EXPOSURE ON RAT THYMOCYTE DEVELOPMENT [J].
CHIAPPELLI, F ;
TIO, D ;
TRITT, SH ;
PILATI, ML ;
TAYLOR, AN .
ALCOHOL, 1992, 9 (06) :481-487
[9]  
COOK RT, 1990, ALCOHOL ALCOHOLISM, V25, P33
[10]   ETHANOL ALTERS ASTROCYTE DEVELOPMENT - A STUDY OF CRITICAL PERIODS USING PRIMARY CULTURES [J].
GUERRI, C ;
SAEZ, R ;
SANCHOTELLO, M ;
DEAQUILERA, EM ;
RENAUPIQUERAS, J .
NEUROCHEMICAL RESEARCH, 1990, 15 (05) :559-565