CRITERIA FOR DETECTION OF CLOSTRIDIUM-DIFFICILE TOXIN PRODUCTION BY COUNTERIMMUNOELECTROPHORESIS

被引:5
作者
RENNIE, RP [1 ]
ELLIOTT, JM [1 ]
NARDINI, MA [1 ]
THORNLEY, JH [1 ]
机构
[1] HENDERSON GEN HOSP, DEPT MICROBIOL, HAMILTON L8V 1C3, ONTARIO, CANADA
关键词
D O I
10.1128/JCM.20.5.923-926.1984
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Counter immunoelectrophoresis (CIE) was compared with a cytotoxicity assay and isolation of toxin-producing C. difficile for the diagnosis of antibiotic-associated diarrhea. Standardized criteria for CIE positivity were established as 2 major precipitin lines with specific C. difficile antitoxin. Using these criteria on 425 fecal specimens, the sensitivity of CIE (80%) was determined to be comparable to that of the cytotoxicity assay (82%). Both methods were highly specific. With carefully controlled techniques, CIE can be used in routine diagnostic laboratories to screen for toxigenic C. difficile.
引用
收藏
页码:923 / 926
页数:4
相关论文
共 19 条
[1]  
ANHALT JP, 1978, DETECTION MICROBIAL
[2]   SIMPLIFIED PROCEDURE FOR THE ROUTINE ISOLATION OF CLOSTRIDIUM-DIFFICILE FROM FECES [J].
BORRIELLO, SP ;
HONOUR, P .
JOURNAL OF CLINICAL PATHOLOGY, 1981, 34 (10) :1124-1127
[3]   CYTO-TOXICITY ASSAY IN ANTIBIOTIC-ASSOCIATED COLITIS [J].
CHANG, TW ;
LAUERMANN, M ;
BARTLETT, JG .
JOURNAL OF INFECTIOUS DISEASES, 1979, 140 (05) :765-770
[4]   NEUTRALIZATION OF CLOSTRIDIUM DIFFICILE TOXIN BY CLOSTRIDIUM SORDELLII ANTI-TOXINS [J].
CHANG, TW ;
GORBACH, SL ;
BARTLETT, JB .
INFECTION AND IMMUNITY, 1978, 22 (02) :418-422
[5]  
Chen J. -S., 1977, ANAEROBE LAB MANUAL, VFourth
[6]  
EHRICH M, 1980, INFECT IMMUN, V28, P1041
[7]  
GEORGE WL, 1979, J CLIN MICROBIOL, V9, P214
[8]   COUNTERIMMUNOELECTROPHORESIS VS CYTO-TOXICITY ASSAY FOR THE DETECTION OF CLOSTRIDIUM-DIFFICILE TOXIN [J].
LEVINE, HG ;
KENNEDY, M ;
LAMONT, JT .
JOURNAL OF INFECTIOUS DISEASES, 1982, 145 (03) :398-398
[9]   RAPID DETECTION AND PRESUMPTIVE IDENTIFICATION OF CLOSTRIDIUM-DIFFICILE BY P-CRESOL PRODUCTION ON A SELECTIVE MEDIUM [J].
PHILLIPS, KD ;
ROGERS, PA .
JOURNAL OF CLINICAL PATHOLOGY, 1981, 34 (06) :642-644
[10]   DETECTION OF CLOSTRIDIUM-DIFFICILE TOXIN BY COUNTERIMMUNOELECTROPHORESIS - A NOTE OF CAUTION [J].
POXTON, IR ;
BYRNE, MD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1981, 14 (03) :349-349