CONSTRUCTION AND REGIONAL LOCALIZATION OF CLONES FROM A NOTI LINKING LIBRARY FROM HUMAN CHROMOSOME-17Q

被引：14

作者：

BORROW, J ^{[1
]}

BLACK, DM ^{[1
]}

GODDARD, AD ^{[1
]}

YAGLE, MK ^{[1
]}

FRISCHAUF, AM ^{[1
]}

SOLOMON, E ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,MOLEC ANAL MAMMALIAN MUTAT LAB,LONDON WC2A 3PX,ENGLAND

来源：

GENOMICS | 1991年 / 10卷 / 02期

关键词：

D O I：

10.1016/0888-7543(91)90335-C

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A NotI linking library was constructed from a somatic cell hybrid containing chromosome 17q as its only human material. A total of 112 human clones were assigned to nine regions of 17q using a somatic cell hybrid mapping panel. The library includes clones that detect the acute promyelocytic leukemia and von Recklinghausen neurofibromatosis translocation breakpoints at 17q11.2-12 and 17q11.2, respectively, on pulsed-field gel electrophoresis. The mapped clones represent over 50% of the estimated number of NotI sites on 17q, and therefore constitute an important resource for long-distance mapping. © 1991.

引用

页码：477 / 480

页数：4

共 21 条

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