KI-RAS ACTIVATION IN PANCREATIC CARCINOMAS OF SYRIAN-HAMSTERS INDUCED BY N-NITROSOBIS(2-HYDROXYPROPYL)AMINE

被引：22

作者：

USHIJIMA, T

TSUTSUMI, M

SAKAI, R

ISHIZAKA, Y

TAKAKU, F

KONISHI, Y

TAKAHASHI, M

SUGIMURA, T

NAGAO, M

机构：

[1] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 3,BUNKYO KU,TOKYO 113,JAPAN

[2] NARA MED UNIV,CTR CANC,DEPT ONCOL PATHOL,KASHIHARA,NARA 634,JAPAN

[3] NATL INST HYG SCI,DIV PATHOL,SETAGAYA KU,TOKYO 158,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1991年 / 82卷 / 09期

关键词：

KI-RAS; HAMSTER; PANCREATIC CARCINOMA; N-NITROSOBIS(2-HYDROXYPROPYL)AMINE;

D O I：

10.1111/j.1349-7006.1991.tb01928.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Five pancreatic carcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki-ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki-ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G-to-A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N-nitrosobis(2-oxopropyl)amine.

引用

页码：965 / 968

页数：4

共 21 条

[1] MOST HUMAN CARCINOMAS OF THE EXOCRINE PANCREAS CONTAIN MUTANT C-K-RAS GENES [J].