NERVE GROWTH-FACTOR MESSENGER-RNA IS EXPRESSED BY GABAERGIC NEURONS IN RAT HIPPOCAMPUS

被引：55

作者：

LAUTERBORN, JC ^{[1
]}

TRAN, TMD ^{[1
]}

ISACKSON, PJ ^{[1
]}

GALL, CM ^{[1
]}

机构：

[1] MAYO CLIN,DEPT BIOCHEM & MOLEC BIOL,JACKSONVILLE,FL 32224

来源：

NEUROREPORT | 1993年 / 5卷 / 03期

关键词：

NERVE GROWTH FACTOR; GLUTAMIC ACID DECARBOXYLASE; HIPPOCAMPUS; BASAL FOREBRAIN; SEPTUM; AGING; IN SITU HYBRIDIZATION;

D O I：

10.1097/00001756-199312000-00023

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

ISOTOPIC and colormetric in situ hybridization techniques were combined to determine if nerve growth factor (NGF) mRNA is colocalized with mRNA for the GABA biosynthetic enzyme glutamic acid decarboxylase (GAD) in adult rat hippocampus. Quantification of neurons labeled with both S-35-labeled GAD(67) mRNA and digoxigenin-labeled NGF cRNA determined that of the NGF cRNA-labeled neurons, 97% within regions CA3-CA1, and 88% within the hilus, were also labeled with GAD(67) cRNA. Overall, 47% of the total population of GAD(67) cRNA labeled cells were NGF cRNA positive. The greater portion of stratum granulosum was lightly labeled by the NGF cRNA alone. The results indicate that, excepting stratum granulosum, NGF is predominantly synthesized by GABAergic neurons in rat hippocampus.

引用

页码：273 / 276

页数：4

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