2 STRUCTURAL GENES ARE ENCODING MALATE SYNTHASE ISOENZYMES IN SACCHAROMYCES-CEREVISIAE

被引：15

作者：

FERNANDEZ, E ^{[1
]}

FERNANDEZ, M ^{[1
]}

RODICIO, R ^{[1
]}

机构：

[1] UNIV OVIEDO,FAC MED,AREA BIOQUIM & BIOL MOLEC,DEPT BIOL FUNCIONAL,E-33006 OVIEDO,SPAIN

来源：

FEBS LETTERS | 1993年 / 320卷 / 03期

关键词：

MALATE SYNTHASE; GLYOXYLATE PATHWAY; ALLANTOIN METABOLISM; GENE DISRUPTION;

D O I：

10.1016/0014-5793(93)80601-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the isolation of a gene encoding yeast malate synthase. A yeast genomic library was screened using a probe homologous to the yeast enzyme obtained by the polymerase chain reaction. The nucleotide sequence of the cloned gene was determined. Computer analysis showed that the isolated gene is identical to the one previously described as DAL7, which is involved in allantoin metabolism [Mol. Cell. Biol. 9 (1989) 3231-3243]. Enzymatic activities of multicopy transformants, Southern analysis and disruption mutants predict the existence of two genes encoding malate synthases that are differentially regulated at the transcriptional level.

引用

页码：271 / 275

页数：5

共 20 条

[1] CLONING AND SEQUENCING OF THE MALATE SYNTHASE GENE FROM HANSENULA-POLYMORPHA [J].

BRUINENBERG, PG ;

BLAAUW, M ;

KAZEMIER, B ;

AB, G .

YEAST, 1990, 6 (03) :245-254

[2] NUCLEOTIDE-SEQUENCE OF THE ACEB GENE ENCODING MALATE SYNTHASE-A IN ESCHERICHIA-COLI [J].

BYRNE, C ;

STOKES, HW ;

WARD, KA .

NUCLEIC ACIDS RESEARCH, 1988, 16 (19) :9342-9342

[3]

COMAI L, 1989, J BIOL CHEM, V264, P2778

[4]

DIXON GH, 1959, BIOCHEM J, V72, pP3

[5]

DUNTZE W, 1969, EUR J BIOCHEM, V10, P83

[6] LARGE-SCALE PURIFICATION AND SOME PROPERTIES OF MALATE SYNTHASE FROM BAKERS-YEAST [J].

DURCHSCHLAG, H ;

BIEDERMANN, G ;

EGGERER, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 114 (02) :255-262

[7] THE MALATE SYNTHASE GENE OF CUCUMBER [J].

GRAHAM, IA ;

SMITH, LM ;

BROWN, JWS ;

LEAVER, CJ ;

SMITH, SM .

PLANT MOLECULAR BIOLOGY, 1989, 13 (06) :673-684

[8]

MALOY SR, 1982, J BACTERIOL, V148, P83

[9]

MORENO E, 1992, EUR J BIOCHEM, V204, P983

[10] PUMPKIN MALATE SYNTHASE - CLONING AND SEQUENCING OF THE CDNA AND NORTHERN BLOT ANALYSIS [J].

MORI, H ;

TAKEDAYOSHIKAWA, Y ;

HARANISHIMURA, I ;

NISHIMURA, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 197 (02) :331-336

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