REPEATED USE OF BACILLUS-SUBTILIS CELL-WALLS FOR COPPER-BINDING

被引：18

作者：

MCLEAN, RJC

CAMPBELL, AM

KHU, PT

PERSAUD, AT

BICKERTON, LE

BEAUCHEMIN, D

机构：

[1] QUEENS UNIV,DEPT CHEM ENGN,KINGSTON K7L 3N6,ONTARIO,CANADA

[2] QUEENS UNIV,DEPT MIN ENGN,KINGSTON K7L 3N6,ONTARIO,CANADA

[3] QUEENS UNIV,DEPT CHEM,KINGSTON K7L 3N6,ONTARIO,CANADA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1994年 / 10卷 / 04期

关键词：

BACILLUS-SUBTILIS; BIOREMEDIATION; COPPER; GRAM-POSITIVE WALLS;

D O I：

10.1007/BF00144475

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Purified cell walls from Bacillus subtilis were repeatedly suspended in 5 mm CuCl2 and, after removing unbound Cu, were suspended in 1% (v/v) HNO3 to release bound Cu. The walls were then regenerated by washing in H2O. After five cycles, copper binding actually increased slightly, probably due to enhanced exposure of binding sites in the walls. Thus bacterial walls may be used repeatedly for metal removal during bioremediation of heavy metal pollution.

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页码：472 / 474

页数：3

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