A BIOMIMETIC MODEL FOR VANADIUM-CONTAINING BROMOPEROXIDASE

被引：21

作者：

SAKURAI, H

TSUCHIYA, K

机构：

[1] Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima, 770

来源：

FEBS LETTERS | 1990年 / 260卷 / 01期

关键词：

Biomimetic model; Bromoperoxidase; Electron spin resonance; Oxygen consumption; Spin-trapping; Vanadium;

D O I：

10.1016/0014-5793(90)80079-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A biomimetic model of vanadium-containing bromoperoxidase (BPO) was proposed. The vanadium-containing BPO possesses vanadate ion (+5 oxidation state) as a prosteric group and catalyses the formation of a carbon-bromide bond in the presence of hydrogen peroxide, bromide ion and bromide acceptor. The reaction mechanism is as yet unknown. Several vanadyl (VO2+, +4 oxidation state) complexes with oxalate, glutarate, succinate, malonate and acetate ligands showed similar bromination activity to that of BPO. Especially, the vanadyl-oxalato complex provided the highest BPO-like activity. Both vanadate ion and vanadyl complexes with nitrogen-containing ligands such as EDTA, glycine and serine showed essentially no BPO-like activity. Thus, the vanadyl-oxalato complex is proposed to be not only a possible chemical model of BPO, but a useful compound to elucidate the reaction mechanism of BPO. © 1990.

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页码：109 / 112

页数：4

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