NICKEL RESISTANCE OF ALCALIGENES-DENITRIFICANS STRAIN-4A-2 IS CHROMOSOMALLY CODED

被引：21

作者：

KAUR, P ^{[1
]}

ROSS, K ^{[1
]}

SIDDIQUI, RA ^{[1
]}

SCHLEGEL, HG ^{[1
]}

机构：

[1] INST MICROBIOL,GRISEBACHSTR 8,W-3400 GOTTINGEN,GERMANY

来源：

ARCHIVES OF MICROBIOLOGY | 1990年 / 154卷 / 02期

关键词：

Alcaligenes denitrificans; Alcaligenes eutrophus; Hydrogenase; Nickel resistance;

D O I：

10.1007/BF00423322

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A newly isolated aerobic hydrogen-oxidizing bacterium, Alcaligenes denitrificans strain 4a-2, differs from related autotrophic bacteria by containing only a single cytoplasmic, NAD-reducing hydrogenase, and by its high resistance to nickel ions, i.e. tolerance to 20 mM NiCl2. Strain 4a-2 harbors a single plasmid of about 250 kb. On helper-assisted mating of 4a-2 with Alcaligenes eutrophus strains H16,G29, and M85 nickelresistant transconjugants were selected; these did not contain the donor plasmid, however. All three transconjugants tolerated 3 to 10 mM NiCl2. The resistance was constitutively expressed. DNA/DNA hybridization showed homology with EcoRI-digested DNA of the wild type 4a-2 and transconjugants using a DNA probe containing nickel resistance genes of pMOL28. This indicated that the 4a-2 nickel resistance genes are located on the chromosome. © 1990 Springer-Verlag.

引用

页码：133 / 138

页数：6

共 26 条

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