HUMAN CATALASE IS IMPORTED AND ASSEMBLED IN PEROXISOMES OF SACCHAROMYCES-CEREVISIAE

被引：5

作者：

DEHOOP, MJ ^{[1
]}

HOLTMAN, WL ^{[1
]}

AB, G ^{[1
]}

机构：

[1] UNIV GRONINGEN, BIOCHEM LAB, 9700 AB GRONINGEN, NETHERLANDS

来源：

YEAST | 1993年 / 9卷 / 01期

关键词：

ASSEMBLY; HUMAN CATALASE; PEROXISOME; TARGETING; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1002/yea.320090108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To study the conservation of peroxisomal targeting signals, we have determined the intracellular localization of human peroxisomal catalase when expressed in yeast. Using immunofluorescence, differential centrifugation and immuno-electron microscopy, we show that the protein is targeted to the peroxisomes of the heterologous cell and assembled in its active tetrameric form. These data show the conservation of the catalase targeting signal and import specificity between human and yeast peroxisomes.

引用

页码：59 / 69

页数：11

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