VASODILATOR RESPONSE OF MESENTERIC ARTERIOLES TO HISTAMINE IN SPONTANEOUSLY HYPERTENSIVE RATS

被引:26
作者
SUZUKI, H
ZWEIFACH, BW
SCHMIDSCHONBEIN, GW
机构
[1] UNIV CALIF SAN DIEGO, DEPT BIOENGN, LA JOLLA, CA 92093 USA
[2] UNIV CALIF SAN DIEGO, INST BIOMED ENGN, LA JOLLA, CA 92093 USA
关键词
MICROCIRCULATION; HISTAMINE; SODIUM NITROPRUSSIDE; ENDOTHELIUM; ACETYLCHOLINE;
D O I
10.1161/01.HYP.26.3.397
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
Recent evidence suggests that spontaneously hypertensive rats (SHR) exhibit an impaired response to inflammatory mediators. We designed this study to analyze the response of arterioles of SHR after stimulation with a proinflammatory agent, histamine, compared with the response of arterioles of normotensive Wistar-Kyoto (WKY) controls. We observed mesenteric arterioles by intravital microscopy in rats under general anesthesia and measured their lumen diameters after histamine superfusion. To compare the concentration-response curve with histamine, we also studied the effect of an endothelium-dependent vasodilator: acetylcholine, and an independent vasodilator, sodium nitroprusside. At the end of each experiment we applied papaverine topically to determine the maximal diameter for each vessel, from which we computed arteriolar tone. Arteriolar tone in SHR is set at a higher steady state level than in WKY. The concentration required for a 50% dilator response (EC50) of histamine in SHR was significantly higher than that in WKY. In SHR the arteriolar response showed the same refractory pattern to histamine as to acetylcholine. In contrast, the EC50 of sodium nitroprusside in SHR was similar to that in WKY. Our results indicate that SHR exhibit an impaired dilator response to histamine that is due to a blunted endothelium-dependent vasodilation.
引用
收藏
页码:397 / 400
页数:4
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