SENSITIVITY TO PLATING OF ESCHERICHIA-COLI-CELLS EXPRESSING THE CRYA GENE FROM BACILLUS-THURINGIENSIS VAR ISRAELENSIS

被引:21
作者
DOUEK, J [1 ]
EINAV, M [1 ]
ZARITSKY, A [1 ]
机构
[1] BEN GURION UNIV NEGEV,DEPT LIFE SCI,IL-84105 BEER SHEVA,ISRAEL
来源
MOLECULAR & GENERAL GENETICS | 1992年 / 232卷 / 01期
关键词
BACILLUS-THURINGIENSIS VAR ISRAELENSIS; MOSQUITO LARVICIDAL DELTA-ENDOTOXIN; T7 RNA POLYMERASE; CYTA GENE PRODUCT; INHIBITION OF DNA SYNTHESIS;
D O I
10.1007/BF00299149
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The gene (cytA) coding for the 27 kDa polypeptide of the Bacillus thuringiensis var. israelensis mosquito larvicidal delta-endotoxin, was cloned into a plasmid containing the T7 bacteriophage promoter. The plasmid was used to transform an Escherichia coli strain containing the T7 RNA polymerase gene 1, under the control of lacP. Loss of colony-forming ability without substantial lysis, associated with immediate inhibition of DNA synthesis, was observed after induction of transformed cells. The cytA gene product may kill E. coli cells by disrupting their chromosome replicating apparatus.
引用
收藏
页码:162 / 165
页数:4
相关论文
共 16 条
[1]   A 20-KILODALTON PROTEIN IS REQUIRED FOR EFFICIENT PRODUCTION OF THE BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS 27-KILODALTON CRYSTAL PROTEIN IN ESCHERICHIA-COLI [J].
ADAMS, LF ;
VISICK, JE ;
WHITELEY, HR .
JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :521-530
[2]   SOME UNIQUE PROPERTIES OF DEOXYRIBONUCLEIC ACID BEARING PORTION OF BACTERIAL MEMBRANE [J].
BALLESTA, JP ;
SCHAECHTER, M ;
SILVERSTEIN, JL ;
SUSSKIND, MM ;
CUNDLIFFE, E ;
DANIELS, MJ .
JOURNAL OF BACTERIOLOGY, 1972, 112 (01) :195-+
[3]   REPLISOME-CONTROLLED INITIATION OF DNA REPLICATION [J].
BLEECKEN, S .
JOURNAL OF THEORETICAL BIOLOGY, 1971, 32 (01) :81-&
[4]  
BLUMENSTEIN S, 1990, CURRENT RES PHOTOSYN, V3, P193
[5]   ULTRASTRUCTURE, PHYSIOLOGY, AND BIOCHEMISTRY OF BACILLUS-THURINGIENSIS [J].
BULLA, LA ;
BECHTEL, DB ;
KRAMER, KJ ;
SHETHNA, YI ;
ARONSON, AI ;
FITZJAMES, PC .
CRC CRITICAL REVIEWS IN MICROBIOLOGY, 1980, 8 (02) :147-204
[6]   STUDIES ON UNBALANCED GROWTH IN ESCHERICHIA-COLI [J].
COHEN, SS ;
BARNER, HD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1954, 40 (10) :885-893
[7]  
Cronan Jr. J.E., 1987, ESCHERICHIA COLI SAL, P474
[8]   RECOVERY FROM THYMINELESS DEATH IN ESCHERICHIA COLI 15T- [J].
DONACHIE, WD ;
HOBBS, DG .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 29 (02) :172-&
[9]  
DOUEK J, 1990, IN PRESS 1ST P AS PA, P1
[10]   CELL-MEMBRANE INTERACTION OF BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS CYTOLYTIC TOXINS [J].
GILL, SS ;
SINGH, GJP ;
HORNUNG, JM .
INFECTION AND IMMUNITY, 1987, 55 (05) :1300-1308