A THERMOSPRAY MASS-SPECTROMETRIC ASSAY FOR FE-INDUCED 4-HYDROXYNONENAL IN TISSUES

被引：12

作者：

BLANCHFLOWER, WJ

WALSH, DM

KENNEDY, S

KENNEDY, DG

机构：

[1] Veterinary Sciences Division, Department of Agriculture For Northern Ireland, Belfast, BT4 3SD, Stormont

来源：

LIPIDS | 1993年 / 28卷 / 03期

关键词：

D O I：

10.1007/BF02536650

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is presented for the determination of 4-hydroxy-nonenal (HNE) in tissue homogenates following in vitro lipid peroxidation induced by iron (Fe++). HNE is measured as the pentafluorobenzyl oxime derivative using liquid chromatography thermospray mass spectrometry. In vitro metabolism of HNE via the glutathione/glutathione-S-transferase pathway was inhibited using iodoacetic and iodobenzoic acids. The assay has been used as an indicator of the peroxidizability of tissue samples from animals both adequate in and depleted of alpha-tocopherol. The concentrations of HNE produced in tissues taken from animals depleted of alpha-tocopherol were found to be up to 8 times higher than those taken from animals supplemented with alpha-tocopherol.

引用

页码：261 / 264

页数：4

共 28 条

[1] 4-HYDROXYALK-2-ENALS ARE SUBSTRATES FOR GLUTATHIONE TRANSFERASE [J].

ALIN, P ;

DANIELSON, UH ;

MANNERVIK, B .

FEBS LETTERS, 1985, 179 (02) :267-270

[2]

BLANCHFLOWER WJ, 1990, SPECTROSC INT, V2, P37

[3]

BULKLEY GB, 1983, SURGERY, V94, P407

[4]

CURZIO M, 1990, INT J IMMUNOTHER, V6, P13

[5] METHODS FOR DETERMINATION OF ALDEHYDIC LIPID-PEROXIDATION PRODUCTS [J].

ESTERBAUER, H ;

ZOLLNER, H .

FREE RADICAL BIOLOGY AND MEDICINE, 1989, 7 (02) :197-203

[6] REACTION OF CYSTEINE WITH ALPHA,BETA-UNSATURATED ALDEHYDES [J].

ESTERBAUER, H ;

ERTL, A ;

SCHOLZ, N .

TETRAHEDRON, 1976, 32 (02) :285-289

[7]

ESTERBAUER H, 1975, Z NATURFORSCH C, V30, P466

[8] MONITORING LIPID-PEROXIDATION BY BREATH ANALYSIS - ENDOGENOUS HYDROCARBONS AND THEIR METABOLIC ELIMINATION [J].

FRANK, H ;

HINTZE, T ;

BIMBOES, D ;

REMMER, H .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1980, 56 (03) :337-344

[9] RAPID HEADSPACE GAS-CHROMATOGRAPHY OF HEXANAL AS A MEASURE OF LIPID-PEROXIDATION IN BIOLOGICAL SAMPLES [J].

FRANKEL, EN ;

HU, ML ;

TAPPEL, AL .

LIPIDS, 1989, 24 (11) :976-981

[10]

ISHIKAWA T, 1986, J BIOL CHEM, V261, P1576

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