DETERMINATION OF GLYCOSYLATION SITES IN O-LINKED GLYCOPEPTIDES - A SENSITIVE MASS-SPECTROMETRIC PROTOCOL

被引：25

作者：

RADEMAKER, GJ

HAVERKAMP, J

THOMASOATES, J

机构：

[1] Bijvoet Center for Biomolecular Research, Department of Mass Spectrometry, Utrecht University, Utrecht, 3508 TB

来源：

ORGANIC MASS SPECTROMETRY | 1993年 / 28卷 / 12期

关键词：

D O I：

10.1002/oms.1210281228

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A sensitive mass spectrometric method for the specific identification of O-glycosylation sites in glycopeptides is presented. Reductive beta-elimination liberates a peptide containing a specifically modified amino acid at the former site of carbohydrate attachment. Mass spectrometry is used to determine the sequence of the modified peptide, in which the position of the modified amino acid marks the site of O-glycosylation. The method also provides information on the carbohydrate chain and is applicable at the low nanomolar level.

引用

页码：1536 / 1541

页数：6

共 25 条

[1] QUANTITATION OF OLIGOSACCHARIDES RELEASED BY THE BETA-ELIMINATION REACTION [J].

AMINOFF, D ;

GATHMANN, WD ;

MCLEAN, CM ;

YADOMAE, T .

ANALYTICAL BIOCHEMISTRY, 1980, 101 (01) :44-53

[2] ENZYMATIC N-GLYCOSYLATION AND O-GLYCOSYLATION OF SYNTHETIC PEPTIDE ACCEPTORS BY DOLICHOL-LINKED SUGAR-DERIVATIVES IN YEAST [J].

BAUSE, E ;

LEHLE, L .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 101 (02) :531-540

[3]

CARLSON DM, 1968, J BIOL CHEM, V243, P616

[4] STRUCTURAL FINGERPRINTING OF ASN-LINKED CARBOHYDRATES FROM SPECIFIC ATTACHMENT SITES IN GLYCOPROTEINS BY MASS-SPECTROMETRY - APPLICATION TO TISSUE PLASMINOGEN-ACTIVATOR [J].

CARR, SA ;

ROBERTS, GD ;

JUREWICZ, A ;

FREDERICK, B .

BIOCHIMIE, 1988, 70 (11) :1445-1454

[5] CARBOHYDRATE MAPPING BY MASS-SPECTROMETRY - A NOVEL METHOD FOR IDENTIFYING ATTACHMENT SITES OF ASN-LINKED SUGARS IN GLYCOPROTEINS [J].

CARR, SA ;

ROBERTS, GD .

ANALYTICAL BIOCHEMISTRY, 1986, 157 (02) :396-406

[6] NOVEL FAST ATOM BOMBARDMENT MASS-SPECTROMETRIC PROCEDURES FOR GLYCOPROTEIN ANALYSIS [J].

DELL, A ;

THOMASOATES, JE ;

ROGERS, ME ;

TILLER, PR .

BIOCHIMIE, 1988, 70 (11) :1435-1444

[7] SPECIFIC METHOD FOR THE FRAGMENTATION OF THE POLYPEPTIDE-CHAIN OF GLYCOPROTEINS - DISTRIBUTION OF CARBOHYDRATE CHAINS ON THE PEPTIDE CORE OF BLOOD-GROUP-SPECIFIC GLYCOPROTEIN [J].

DEREVITSKAYA, VA ;

LIKHOSHERSTOV, LM ;

MARTYNOVA, MD ;

KOCHETKOV, NK .

CARBOHYDRATE RESEARCH, 1983, 120 (AUG) :85-94

[8] BETA-ELIMINATION AND REDUCTION REACTIONS AND SOME APPLICATIONS OF DIMETHYLSULFOXIDE ON SUBMAXILLARY GLYCOPROTEINS [J].

DOWNS, F ;

HERP, A ;

MOSCHERA, J ;

PIGMAN, W .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 328 (01) :182-192

[9]

DOWNS F, 1977, INT J PEPT PROT RES, V10, P315

[10]

DOWNS F, 1979, GLYCOCOJUNGATE RES, V1, P145

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