PLUS-STRAND STRONG-STOP DNA-SYNTHESIS IN RETROTRANSPOSON TY1

被引：27

作者：

LAUERMANN, V ^{[1
]}

NAM, KB ^{[1
]}

TRAMBLEY, J ^{[1
]}

BOEKE, JD ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MOLEC BIOL & GENET,BALTIMORE,MD 21205

来源：

JOURNAL OF VIROLOGY | 1995年 / 69卷 / 12期

关键词：

D O I：

10.1128/JVI.69.12.7845-7850.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Reverse transcription in the yeast retrotransposon Tyl follows the general ''rules'' of retroviral replication overall. However, some details of the retroviral and Tyl reverse transcription processes are different. We have identified and determined the structure of plus-strand strong-stop DNA and examined the effect of polypurine tract deletion mutations on its synthesis. Furthermore, we have defined the stop signal for plus strand strong-stop DNA synthesis as an unusual 2'-O-ribosylated nucleotide in the primer tRNA. Full-length plus-strand strong-stop DNA, following strand transfer, would have a terminal 2-base mismatch with minus-strand DNA. These findings indicate that the mechanism of plus-strand strong-stop DNA transfer in Tyl differs from that of the retroviral transfer and suggest that full-length plus-strand strong-stop DNA is not a direct intermediate in Tyl retrotransposition.

引用

页码：7845 / 7850

页数：6