USE OF A RAPID MICROBIAL ATP BIOLUMINESCENCE ASSAY TO DETECT CONTAMINATION ON BEEF AND PORK CARCASSES

被引：59

作者：

SIRAGUSA, GR

CUTTER, CN

DORSA, WJ

KOOHMARAIE, M

机构：

[1] United States Department of Agriculture, Agricultural Research Service, United States Meat Animal Research Center, Clay Center, NE 68933

来源：

JOURNAL OF FOOD PROTECTION | 1995年 / 58卷 / 07期

关键词：

ATP BIOLUMINESCENCE; RAPID MICROBIAL TESTING; HACCP MONITORING;

D O I：

10.4315/0362-028X-58.7.770

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new microbial ATP bioluminescence assay was shown to be an accurate and rapid method to determine the levels of generic bacterial contamination on beef (n = 400) and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of > log(10) 2.0 aerobic CFU/cm(2) on beef carcasses and of > log(10) 3.2 aerobic CFU/cm(2) for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.

引用

页码：770 / 775

页数：6

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