Crystallization of rat liver macrophage migration inhibitory factor for MAD analysis

被引：19

作者：

Sugimoto, H ^{[1
]}

Suzuki, M ^{[1
]}

Nakagawa, A ^{[1
]}

Tanaka, I ^{[1
]}

Fujinaga, M ^{[1
]}

Nishihira, J ^{[1
]}

机构：

[1] HOKKAIDO UNIV,SCH MED,CENT RES INST,SAPPORO,HOKKAIDO 060,JAPAN

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1995年 / 115卷 / 03期

关键词：

D O I：

10.1006/jsbi.1995.1057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recombinant macrophage migration inhibitory factor (MIF) of a rat liver was crystallized using the hanging-drop vapor diffusion method. We also crystallized a selenomethionyl rat MIF under similar conditions for X-ray structure analysis using the multiwavelength anomalous diffraction method. Furthermore, two kinds of selenomethionyl rat MIF mutants, in which one or two of three methionine residues were replaced by alanine [(Met101Ala), (Met2Ala and Met101Ala)], were constructed and crystallized. These crystals belong to two different forms of the hexagonal space group P6(3). The first form has cell dimensions of a = b = 95.7 Angstrom and c = 87.9 Angstrom and contains two dimers in an asymmetric unit, while the second form, whose cell dimensions are a = b = 61.8 Angstrom and c = 53.4 Angstrom, contains only one subunit in an asymmetric unit. Preliminary X-ray examination shows that these crystals are suitable for high-resolution structure analysis. (C) 1995 Academic Press, Inc.

引用

页码：331 / 334

页数：4

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