CHARACTERIZATION OF ANTIBODIES AGAINST HUMAN N-TERMINAL PARATHYROID-HORMONE BY EPITOPE MAPPING

被引：8

作者：

TAMPE, J

BROSZIO, P

MANNECK, HE

MISSBICHLER, A

BLIND, E

MULLER, KB

SCHMIDTGAYK, H

ARMBRUSTER, FP

机构：

[1] BIOMED,A-1210 VIENNA,AUSTRIA

[2] UNIV HEIDELBERG,DEPT INTERNAL MED 1,W-6900 HEIDELBERG,GERMANY

[3] ARZTL LABORGEMEINSCHAFT,W-6900 HEIDELBERG,GERMANY

来源：

JOURNAL OF IMMUNOASSAY | 1992年 / 13卷 / 01期

关键词：

D O I：

10.1080/15321819208019821

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two polyclonal antisera from goat and mouse and two monoclonal antibodies against human parathyroid hormone (1-34) were characterised by epitope mapping. Hexapeptides were synthesized on polystyrene pins, the sequences of which overlapped and represented the entire sequence of hPTH(1-34). Binding of antibodies to these hexapeptides was determined and antigenic determinants thus characterized. At least one predominant binding sequence was detected in the region of hPTH(7-14).

引用

页码：1 / 13

页数：13

共 14 条

[1]

ATKINSON M, 1982, Journal of Immunoassay, V3, P31, DOI 10.1080/15321818208056985

[2]

BLIND E, 1987, CLIN CHEM, V33, P1376

[3] HUMAN PARATHYROID-HORMONE - IMMUNOLOGICAL CHARACTERIZATION OF ANTIBODIES AGAINST A GLANDULAR EXTRACT AND SYNTHETIC AMINO-TERMINAL FRAGMENTS 1-12 AND 1-34 AND THEIR USE IN DETERMINATION OF IMMUNOREACTIVE HORMONE IN HUMAN SERA [J].