A NOVEL, HIGHLY EFFICIENT GENE-CLONING SYSTEM FOR MICROMONOSPORA STRAINS

被引：18

作者：

HASEGAWA, M

DAIRI, T

OHTA, T

HASHIMOTO, E

机构：

[1] Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Tokyo 194, 3-6-6 Asahi-machi, Machida-shi

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 21期

关键词：

D O I：

10.1128/jb.173.21.7004-7011.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A highly efficient gene-cloning system for Micromonospora olivasterospora, a producer of the antibiotic fortimicin A (astromicin), suited to shotgun cloning has been developed. The system is supported by two new advancements accomplished in this study. One is the construction of novel plasmid vectors pMO116, pMO126, pMO133, pMO136, and pMO217, all consisting of replicons from newly found Micromonospora plasmids and selectable markers cloned from a neomycin-producing Micromonospora strain. The other advancement is the establishment of a new protocol for bacterial protoplasting in which some kinds of sugar alcohols are added in precultures. Such sugar alcohols were found to sensitize a wide taxonomical range of bacteria to lysozyme. The system is reproducible and reliable and has a high efficiency of more than 10(6) CFU/mu-g of DNA.

引用

页码：7004 / 7011

页数：8

共 26 条

[1] TRANSCRIPTION FROM THE P1-PROMOTERS OF MICROMONOSPORA-ECHINOSPORA IN THE ABSENCE OF NATIVE UPSTREAM DNA-SEQUENCES [J].