IN-SITU HYBRIDIZATION FOR THE IDENTIFICATION OF HELICOBACTER-PYLORI IN PARAFFIN WAX EMBEDDED TISSUE

被引：19

作者：

BASHIR, MS

LEWIS, FA

QUIRKE, P

LEE, A

DIXON, MF

机构：

[1] GEN INFIRM,ACAD UNIT PATHOL,LEEDS,W YORKSHIRE,ENGLAND

[2] UNIV NEW S WALES,DEPT MICROBIOL,SYDNEY,NSW,AUSTRALIA

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1994年 / 47卷 / 09期

关键词：

D O I：

10.1136/jcp.47.9.862

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A method for identifying Helicobacter pylori using a non-isotopic in situ hybridisation technique is described. A probe generated by polymerase chain reaction (PCR) with primers directed against parts of the Helicobacter pylori 16SrRNA sequence was used. Paraffin wax embedded gastric biopsy specimens from patients with and without gastritis were hybridised with the probe, and the method was shown to be sensitive and specific for H pylori.

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页码：862 / 864

页数：3

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