SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR NIFEDIPINE IN HUMAN PLASMA UTILIZING ULTRAVIOLET DETECTION

被引:42
作者
GRUNDY, JS [1 ]
KHERANI, R [1 ]
FOSTER, RT [1 ]
机构
[1] UNIV ALBERTA,DIV PHARM & PHARMACEUT SCI,EDMONTON T6G 2N8,ALBERTA,CANADA
来源
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 654卷 / 01期
关键词
D O I
10.1016/0378-4347(93)E0449-Z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid, simple, sensitive and selective reversed-phase high-performance liquid chromatographic (HPLC) technique is reported for the determination of nifedipine in human plasma. The procedure involves extraction of nifedipine from plasma under alkaline conditions (pH 12), separation via reversed-phase HPLC and ultraviolet detection (350 nm). The peak corresponding to nifedipine was free of interference from its photodegradation products or metabolites. The method was validated over the range 5-250 ng/ml nifedipine using weighted least-squares linear regression analysis. Accuracy and precision were within approximately 10% or less over the concentration range, except for the lowest concentration point which, nonetheless, was acceptable and approached 15%. The minimum quantifiable concentration of nifedipine was determined to be 5 ng/ml. The minimum detectable concentration was in the order of 1 ng/ml. Analysis of plasma samples collected from healthy volunteers demonstrate that this assay is applicable to clinical and pharmacokinetic studies.
引用
收藏
页码:146 / 151
页数:6
相关论文
共 19 条
[1]   OCCURRENCE AND MEASUREMENT OF NIFEDIPINE AND ITS NITROPYRIDINE DERIVATIVE IN HUMAN-BLOOD PLASMA [J].
DOKLADALOVA, J ;
TYKAL, JA ;
COCO, SJ ;
DURKEE, PE ;
QUERCIA, GT ;
KORST, JJ .
JOURNAL OF CHROMATOGRAPHY, 1982, 231 (02) :451-458
[2]   ANALYSIS OF CALCIUM BLOCKER DRUGS IN PLASMA, HRGC AND HPLC ANALYTICAL CONDITIONS FOR PHARMACOKINETIC STUDIES [J].
GIACHETTI, C ;
POLETTI, P ;
ZANOLO, G .
JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1987, 10 (12) :654-658
[3]   QUANTITATIVE-ANALYSIS OF THE DIHYDROPYRIDINES, 3-(2-FUROYL)-5-METHOXYCARBONYL-2,6-DIMETHYL-4-(2-NITROPHENYL)-1,4-DIHYDROPYRIDINE AND NIFEDIPINE, BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION [J].
HUEBERT, ND ;
SPEDDING, M ;
HAEGELE, KD .
JOURNAL OF CHROMATOGRAPHY, 1986, 353 :175-180
[4]   LIQUID-CHROMATOGRAPHIC DETERMINATION OF NIFEDIPINE IN PLASMA AND OF ITS MAIN METABOLITE IN URINE [J].
KLEINBLOESEM, CH ;
VANHARTEN, J ;
VANBRUMMELEN, P ;
BREIMER, DD .
JOURNAL OF CHROMATOGRAPHY, 1984, 308 (JUN) :209-216
[5]   NIFEDIPINE - RELATIONSHIP BETWEEN PHARMACOKINETICS AND PHARMACODYNAMICS [J].
KLEINBLOESEM, CH ;
VANBRUMMELEN, P ;
BREIMER, DD .
CLINICAL PHARMACOKINETICS, 1987, 12 (01) :12-29
[6]   RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NIFEDIPINE IN PLASMA WITH ONLINE PRECOLUMN SOLID-PHASE EXTRACTION [J].
NITSCHE, V ;
SCHUTZ, H ;
EICHINGER, A .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 420 (01) :207-211
[7]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF NIFEDIPINE AND A TRACE PHOTODEGRADATION PRODUCT IN HOSPITAL PRESCRIPTIONS [J].
OHKUBO, T ;
NORO, H ;
SUGAWARA, K .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1992, 10 (01) :67-70
[8]   GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC ANALYSIS OF PLASMA NIFEDIPINE [J].
PATRICK, KS ;
JARVI, EJ ;
STRAUGHN, AB ;
MEYER, MC .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 495 :123-130
[9]   NONLINEAR 1ST-PASS METABOLISM OF NIFEDIPINE IN HEALTHY-SUBJECTS [J].
SCHELLENS, JHM ;
VANHAELST, IMM ;
HOUSTON, JB ;
BREIMER, DD .
XENOBIOTICA, 1991, 21 (04) :547-555
[10]   DETERMINATION OF NIFEDIPINE AND ITS 3 PRINCIPAL METABOLITES IN PLASMA AND URINE BY AUTOMATED ELECTRON-CAPTURE CAPILLARY GAS-CHROMATOGRAPHY [J].
SCHMID, BJ ;
PERRY, HE ;
IDLE, JR .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 425 (01) :107-119