THE REGULATION OF ARACHIDONATE LIPOXYGENASE METABOLITE FORMATION IN CELLS DERIVED FROM INTRAUTERINE TISSUES

被引：8

作者：

EDWIN, SS ^{[1
]}

THAI, D ^{[1
]}

LAMARCHE, S ^{[1
]}

BRANCH, DW ^{[1
]}

MITCHELL, MD ^{[1
]}

机构：

[1] UNIV UTAH,SCH MED,DEPT OBSTET & GYNECOL,SALT LAKE CITY,UT 84132

来源：

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1995年 / 52卷 / 04期

关键词：

D O I：

10.1016/0952-3278(95)90041-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Products of arachidonic acid (AA) metabolism via the lipoxygenase pathways may have key roles in the maintenance of pregnancy and the onset of labor. We have determined whether calcium ionophores can modulate the rate of biosynthesis within the uterus of five important arachidonate lipoxygenase metabolites, i.e. leukotriene B-4 (LTB(4)), LTC(4), 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, and 15-HETE. Amnion, chorion, and decidual cells were isolated, grown to confluence and incubated with ionomycin. The production of LTB(4), LTC(4), 5-HETE, 12-HETE, and 15-HETE was determined using specific radioimmunoassays. Cell-specific, concentration-related stimulatory actions of ionomycin on 5-HETE, 12-HETE, 15-HETE, and LTC(4) but not LTB(4) production were found. A23187 had effects similar to ionomycin. Hence elevation of intracellular calcium levels can result in enhanced intrauterine production of arachidonate lipoxygenase metabolites that may affect pregnancy outcome.

引用

页码：229 / 233

页数：5

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