INTERACTION OF AMIKACIN LOADED NANOPARTICLE WITH PHOSPHATIDYLCHOLINE MONOLAYERS AS MEMBRANE MODELS

Using monolayers as a membrane model, the interaction of free amikacin and amikacin-loaded polybutylcyanoacrylate nanoparticles with phosphatidylcholine (PC) has been investigated. The kinetics of penetration of this drug into the monolayers at 2.5, 5, 10 and 20 mN m-1 were measured and compared with those of free nanoparticles. The stability of amikacin sulphate (AMK)-loaded poly(butyl-2-cyanoacrylate) (PBCA) nanoparticles over a period of 98 days at 25-degrees-C has been studied. The kinetics of their penetration into the lipid monolayer as a membrane model was analysed periodically. Their size and polydispersity index were determined at the same time by photon correlation spectroscopy. A decrease in the penetration of AMK-loaded PBCA nanoparticles on phosphatidylcholine monolayers was observed, this effect being most pronounced during the final weeks, when the loss of surface pressure increases reached 31.59% of the initial value. At the same time, the particle size and polydispersity index increased during the final stages. Neither effect is significant in the first 2 months of the assays.