INVIVO AND INVITRO EFFECTS OF ISRADIPINE ON CYTOSOLIC CA-2+ CONCENTRATION IN ERYTHROCYTES FROM SPONTANEOUSLY HYPERTENSIVE RATS

被引：6

作者：

MORRIS, MJ ^{[1
]}

DAVIDDUFILHO, M ^{[1
]}

DEVYNCK, MA ^{[1
]}

机构：

[1] CHU NECKER,DEPT PHARMACOL,CNRS,URA 1482,156 RUE VAUGIRARD,F-75015 PARIS,FRANCE

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1992年 / 5卷 / 12期

关键词：

ISRADIPINE; CYTOSOLIC CA-2+ CONCENTRATION; ERYTHROCYTE; FURA-2; SPONTANEOUSLY HYPERTENSIVE RAT;

D O I：

10.1093/ajh/5.12.887

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

Cytosolic Ca2+ concentration ([Ca2+]i) was investigated in erythrocytes from spontaneously hypertensive rats (SHR) and their normotensive controls (WKY), after an acute treatment with the Ca2+ antagonist isradipine. Blood samples were obtained from conscious rats and [Ca2+]i measured with fura-2. The [Ca2+]i was higher in SHR than in WKY erythrocytes (P < .05). Isradipine administration had no effect on WKY [Ca2+]i, but reduced that of SHR to WKY levels after 1 h. In vitro, isradipine dose-dependently decreased [Ca2+]i only in SHR (P = .006). The reduction by isradipine of the elevated [Ca2+]i in SHR suggests the presence of a greater dihydropyridine-sensitive Ca2+ influx in the SHR erythrocyte.

引用

页码：887 / 891

页数：5

共 18 条

[1]

Eme P., Hermsmeyer K., Intracellular vascular muscle Ca2+ modulation in genetic hypertension, Hypertension, 14, pp. 145-151, (1989)

[2]

Sada T., Koike H., Ikeda M., Et al., Cytosolic free calcium of aorta in hypertensive rats, Hypertension, 16, pp. 245-251, (1990)

[3]

Le Quan Sang K.H., Benlian P., Kanawati C., Et al., Platelet cytosolic free calcium concentration in primary hypertension, J Hypertens, 3, pp. S33-S36, (1985)

[4]

Oshima T., Young E., Bukoski R.D., McCarron D., Abnormal calcium handling by platelets of spontaneous hypertensive rats, Hypertension, 15, pp. 606-611, (1990)

[5]

Battle D., Janss J., Lapointe M., Et al., Cytosolic calcium in T lymphocytes from spontaneously hypertensive rats, Am J Hypertens, 3, pp. 343-348, (1990)

[6]

Orlov S., Pokudin N., Postnov Y., Calcium transport in erythrocytes of rats with spontaneous hypertension, J Hypertens, 6, pp. 829-837, (1988)

[7]

Striessnig J., Zemig G., Glossmann H., Human red-bloodcell Ca2+-antagonist binding sites: Evidence for an unusual receptor coupled to the nucleoside transporter, Eur J Biochem, 150, pp. 67-77, (1985)

[8]

Engelmann B., Duhm J., Distinction of two components of passive Ca2+ transport into human erythrocytes by Ca2+ entry blockers, Biochim Biophys Acta, 981, pp. 36-42, (1989)

[9]

Halperin J., Brugnara C., Tosteson M., Et al., Voltage-activated cation transport in human erythrocytes, Am J Physiol 199, 257, pp. C986-C996

[10]

David-Dufilho M., Montenay-Garestier T., Devynck M.A., Fluorescence measurements of free Ca2+ concentration in human erythrocytes using the Ca2+-indicator fura-2, Cell Calcium, 9, pp. 167-173, (1988)

← 1 2 →