COMPETITIVE POLYMERASE CHAIN-REACTION ASSAY FOR QUANTITATION OF HIV-1 DNA AND RNA

被引：48

作者：

STIEGER, M ^{[1
]}

DEMOLLIERE, C ^{[1
]}

AHLBORNLAAKE, L ^{[1
]}

MOUS, J ^{[1
]}

机构：

[1] F HOFFMANN LA ROCHE & CO LTD,CENT RES UNIT,CH-4002 BASEL,SWITZERLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 34卷 / 02期

关键词：

POLYMERASE CHAIN REACTION; HIV-1; QUANTITATIVE PCR; VIRUS QUANTIFICATION;

D O I：

10.1016/0166-0934(91)90095-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A quantitative PCR assay for the detection of HIV-1 nucleic acids is described. The assay is based on a competitive internal standard nucleic acid which can be discriminated from target sequences by the presence of a new restriction enzyme site. The method was used to quantitate plasmid molecules containing HIV-1 sequences, HIV-1 DNA and HIV-1 RNA purified from HIV-1-infected tissue culture cells as well as HIV-1 DNA present in the peripheral blood mononuclear cells of an AIDS patient. The assay will be valuable for assessing viral load in AIDS patients and for the study of viral gene expression.

引用

页码：149 / 160

页数：12

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