ANTIGEN-ANTIBODY RECOGNITION BY FOURIER-TRANSFORM IR SPECTROSCOPY ATTENUATED TOTAL REFLECTION STUDIES - BIOTIN AVIDIN COMPLEX AS AN EXAMPLE

被引：27

作者：

BARBUCCI, R ^{[1
]}

MAGNANI, A ^{[1
]}

RONCOLINI, C ^{[1
]}

SILVESTRI, S ^{[1
]}

机构：

[1] SCLAVO SPA, I-53100 SIENA, ITALY

来源：

BIOPOLYMERS | 1991年 / 31卷 / 07期

关键词：

D O I：

10.1002/bip.360310703

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biotin-avidin recognition is studied by Fourier transform ir spectroscopy/attenuated total reflection (FTIR/ATR) under physiological conditions. The ureido portion of biotin is confirmed to be involved in the interaction with avidin, as previously found, but when the biotin-avidin complex forms, an electrostatic interaction occurs between the carboxylate group of the biotin molecule and the protonated aminic end group of the avidin amino acid side chains. Comparison of the biotin-avidin system with the biotin-1,4-diaminobutane and biotin-tryptophan systems confirms these findings.

引用

页码：827 / 834

页数：8

共 11 条

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[2]

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