H-1-NMR STUDY OF ENZYMATICALLY GENERATED WHEAT-ENDOSPERM ARABINOXYLAN OLIGOSACCHARIDES - STRUCTURES OF HEPTA-SACCHARIDES TO TETRADECA-SACCHARIDES CONTAINING 2 OR 3 BRANCHED XYLOSE RESIDUES

The structures of hepta- to tetradeca-saccharides, generated by digestion of wheat-endosperm arabinoxylan with endo-(1 --> 4)-beta-D-xylanase, and isolated by gel-permeation chromatography on Bio-Gel P-6 and high-performance anion-exchange chromatography with pulsed amperometric detection (h.p.a.e.-p.a.d.), were elucidated using monosaccharide and methylation analysis, f.a.b.-m.s., and H-1-n.m.r. spectroscopy. The structures identified had two branching elements, --> 4)[alpha-L-Araf-(1 --> 3)]-beta-D-Xylp-(1 --> and/or --> 4)[alpha-L-Araf-(1 --> 2)][alpha-L-Araf-(1 --> 3)]-beta-D-Xylp-(1 -->, directly connected to each other in all four possible combinations. The h.p.a.e.-p.a.d. elution pattern showed that these combinations are not present in equal amounts. Also, compounds containing two 2,3-branched beta-D-Xylp residues separated by one or two unbranched beta-D-Xylp residues were found, and the presence of a tetradecasaccharide containing three 2,3-branched beta-D-Xylp residues was established.