RAPID AND SIMPLE ISOLATION OF DNA FROM AGAROSE GELS

被引:25
作者
GREY, M [1 ]
BRENDEL, M [1 ]
机构
[1] JW GOETHE UNIV,INST MIKROBIOL,THEODOR STERN KAI 7,HAUS 75,W-6000 FRANKFURT,GERMANY
关键词
DNA ISOLATION; AGAROSE GELS; MOLECULAR CLONING;
D O I
10.1007/BF00351746
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.
引用
收藏
页码:83 / 84
页数:2
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