1-ALPHA,25-DIHYDROXYVITAMIN D-3 RAPIDLY ALTERS PHOSPHOLIPID-METABOLISM IN THE NUCLEAR-ENVELOPE OF OSTEOBLASTS

被引：15

作者：

SORENSEN, AM

BARAN, DT

机构：

[1] Department of Orthopedics, University of Massachusetts Medical Center, Worcester, Massachusetts

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 1995年 / 58卷 / 01期

关键词：

NUCLEAR CALCIUM; INOSITOL TRIPHOSPHATE; OSTEOSARCOMA CELLS; OSTEOBLAST NUCLEI; VITAMIN-D;

D O I：

10.1002/jcb.240580104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1 alpha,25-Dihydroxyvitamin D-3 (1 alpha,25-(OH)(2)D-3) has been shown to increase cytosolic calcium and inositol triphosphate levels in rat osteosarcoma cells (ROS 17/2.8) and to increase nuclear calcium in these cells. To determine the mechanism(s) of 1 alpha,25-(OH)(2)D-3-induced changes in nuclear calcium, the effect of the hormone on phospholipid metabolism in isolated osteoblast nuclei was assessed. 1 alpha,25(OH)(2)D-3, 20 nM, increased inositol triphosphate levels in the nuclei after 5 min of treatment. The biologically inactive epimer, 1 beta,25-(OH)(2)D-3, had no significant effect on inositol triphosphate levels. ATP, 1 mM, also increased inositol triphosphate levels in the isolated nuclei after 5 min. 1 alpha,25-(OH)(2)D-3, 20 nM, increased calcium in the isolated nuclei in the presence but not in the absence of extranuclear calcium within 5 min. Nuclear calcium was also increased within 5 min by ATP, 1 mM, and inositol triphosphate, 1 mM. The effect of ATP on nuclear calcium was not additive with 1 alpha,25-(OH)(2)D-3, suggesting that these two agents increase nuclear calcium in these osteoblast-like cells by similar mechanisms. In summary, 1 alpha,25-(OH)(2)D-3 and ATP rapidly increase inositol triphosphate levels in nuclei isolated from ROS 17/2.8 cells. The hormone, the nucleotide, and the inositol phospholipid increase nuclear calcium. Thus, the 1 alpha,25-(OH)(2)D-3 and ATP effects on nuclear calcium may be mediated by changes in phospholipid metabolism in the nuclei of these osteoblast-like cells. (C) 1995 Wiley-Liss, Inc.

引用

页码：15 / 21

页数：7

共 41 条

[1]

Baran DT, Sorensen AM, Honeyman TW, Rapid actions of 1,25‐dihydroxyvitamin D3 on hepatocyte phospholipids, J Bone Miner Res, 3, pp. 593-600, (1988)

[2]

Baran DT, Kelly AM, Lysophosphatidylinositol: A potential mediator of 1,25‐dihydroxyvitamin D3‐induced increments in hepatocyte cytoslic calcium, Endocrinology, 122, pp. 930-934, (1988)

[3]

Baran DT, Sorensen AM, Honeyman TW, Ray R, Holick MF, Rapid actions of 1α25‐dihydroxyvitamin D3 on Ca+2 and phospolipids in isolated rat liver nuclei, FEBS Lett, 259, pp. 205-208, (1989)

[4]

Baran DT, Sorensen AM, Honeyman TW, Ray R, Holick MF, 1α25‐dihydroxyvitamin D3‐induced increments in hepatocyte cytosolic calcium and lysophosphatidylinositol: inhibition by pertussis toxin and 1α25‐digydroxyvitamen D3, J Bone Miner Res, 5, pp. 517-524, (1990)

[5]

Baran DT, Sorensen AM, Shalhoub V, Owen T, Oberdorf A, Stein G, Lian J, 1α25‐digydroxyvitamin D3 rapidly increases cytosolic calcium in clonal rat osteosarcoma cells lacking the vitamin D receptro, J Bone Miner Res, 6, pp. 1269-1275, (1991)

[6]

Baran DT, Sorensen AM, Shalhoub V, Owen T, Stein G, Lian J, The rapid nongenomic actions of 1α25‐digydroxyvitamin D3 modulate the hormone‐induced increments in osteocalcin gene transcription in osteoblast‐like cells, J Cell Biochem, 50, pp. 124-129, (1992)

[7]

Berezney R, Basler J, Kaplan SC, Hughes BB, The nuclear matrix of slowly and rapidly proliferating liver cells, Eur J Cell Biol, 20, pp. 139-142, (1979)

[8]

Caffery JM, Faracg-Carson MC, Vitamin D3 metabolites modulate dihydropyridine‐sensitive calsium currents in clonal rat osteosarcoma cells, J Biol Chem, 264, pp. 20265-20274, (1989)

[9]

Capitani S, Bertagnolo Y, Mazzoni M, Santi P, Previati M, Antonucci A, Manzoli FA, Lipid phosphorylation in isolated rat liver nuclei synthesis of polyphoosphoinosides at subnuclear level, FEBS Lett, 254, pp. 194-198, (1989)

[10]

Capitani S, Helms B, Mazzoni M, Previati M, Bertagnolo V, Wirtz KWA, Manzoli FA, Uptake and phosphorylation of phosphlatidylinositol by rat liver nuclei: role of phosphatidylinositol transfer protein, Biochem Biophys Acta, 1044, pp. 193-200, (1990)

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