COMPARATIVE-STUDY OF THE GENETIC-VARIABILITY IN THYMIDINE KINASE AND GLYCOPROTEIN-B GENES OF HERPES-SIMPLEX VIRUSES BY THE RNASE-A MISMATCH CLEAVAGE METHOD

被引：3

作者：

ROJAS, JM

DOPAZO, J

SANTANA, M

LOPEZGALINDEZ, C

TABARES, E

机构：

[1] INST SALUD CARLOS 3,CTR NACL BIOL CELULAR & RETROVIRUS,E-28220 MAJADAHONDA,SPAIN

[2] UNIV AUTONOMA MADRID,CSIC,CTR NACL BIOTECNOL,E-28049 MADRID,SPAIN

[3] UNIV AUTONOMA MADRID,FAC MED,DEPT MICROBIOL,E-28049 MADRID,SPAIN

来源：

VIRUS RESEARCH | 1995年 / 35卷 / 02期

关键词：

HERPES SIMPLEX VIRUS; GENETIC VARIABILITY; NASE A METHOD;

D O I：

10.1016/0168-1702(94)00097-V

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The variability of herpes simplex viruses has been measured using the RNAse A mismatch cleavage method in two genes: thymidine kinase and glycoprotein B of both HSV-1 and HSV-2. This technique permitted us to study the variability of the virus with a greater level of resolution than restriction endonuclease analysis. The phylogenetic trees obtained for the different genes allowed us to identify consistent clusters of viruses circulating in the same geographical area. Our results showed that thymidine kinase is more heterogeneous than glycoprotein B for both subtypes of HSV, and confirmed that HSV-1 is more heterogeneous than HSV-2 for both genes. This is the first time that this kind of analysis has been applied to DNA viruses.

引用

收藏

页码：205 / 214

页数：10

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