DIFFERENTIAL CALCIUM-DEPENDENCE IN THE ACTIVATION OF C-JUN KINASE AND MITOGEN-ACTIVATED PROTEIN-KINASE BY MUSCARINIC ACETYLCHOLINE-RECEPTORS IN RAT 1A CELLS

被引：63

作者：

MITCHELL, FM ^{[1
]}

RUSSELL, M ^{[1
]}

JOHNSON, GL ^{[1
]}

机构：

[1] UNIV COLORADO,SCH MED,DEPT PHARMACOL,DENVER,CO 80262

来源：

BIOCHEMICAL JOURNAL | 1995年 / 309卷

关键词：

D O I：

10.1042/bj3090381

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbachol stimulation of the muscarinic acetylcholine m1 receptor (m1R), stably expressed in Rat 1a fibroblasts, resulted in a calcium-dependent activation of c-Jun kinase (JNK). Stimulation of the muscarinic acetylcholine m2 receptor (m2R), stably expressed in Rat la fibroblasts, resulted in a G(i)-mediated activation of JNK that was weak relative to that observed with the m1R. Chelation of calcium inhibited the m2R-mediated activation of JNK but not the robust m2R stimulation of mitogen-activated protein kinase (MARK) activity. These findings demonstrate a role for the second messenger, calcium, in the differential regulation of the activity of JNK and MARK in Rat 1a cells.

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页码：381 / 384

页数：4

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