THE PSBC START CODON IN SYNECHOCYSTIS SP PCC-6803

被引：22

作者：

CARPENTER, SD ^{[1
]}

CHARITE, J ^{[1
]}

EGGERS, B ^{[1
]}

VERMAAS, WFJ ^{[1
]}

机构：

[1] ARIZONA STATE UNIV, CTR STUDY EARLY EVENTS PHOTOSYNTH, TEMPE, AZ 85287 USA

来源：

FEBS LETTERS | 1990年 / 260卷 / 01期

关键词：

CP43; Cyanobacterium; Gene; psbC; Photosystem II; Translation initiation;

D O I：

10.1016/0014-5793(90)80085-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The translation start codon for psbC, the gene encoding CP43, a chlorophyll-binding protein of photosystem II, has been identified for the cyanobacterium Synechosystis sp. PCC 6803 using site-directed mutagenesis. An AUG codon, about 50 bases upstream from the end of psbD-I had previously been assumed to be the translation start site of psbC. However, the fact that the AUG codon is not present in psbC from several other organisms, whereas a GUG codon 14 bases upstream from the end of psbD-I is strictly conserved suggests that CP43 translation starts at the latter codon. Mutation of GUG, but not of AUG, led to a loss of CP43 and photoautotrophic growth, indicating that the GUG codon is the sole initiation site for translation of the CP43 protein in Synechocystis sp. PCC 6803. © 1990.

引用

页码：135 / 137

页数：3

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