DEVELOPMENT OF STABLE, GENETICALLY WELL-DEFINED CONDITIONALLY VIABLE ESCHERICHIA-COLI STRAINS

被引：4

作者：

DEGRYSE, E

机构：

[1] Department of Molecular Biology, Transgene S.A., Strasbourg, F-67000, 11, rue de Molsheim

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 227卷 / 01期

关键词：

ESCHERICHIA-COLI; DAPD GENE; SUBSTITUTION; ANTIBIOTIC RESISTANCE; CLONING;

D O I：

10.1007/BF00260705

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The diaminopimelate (DAP) pathway provides the cell with lysine and with DAP, a vital cell wall constituent. Mutations in the DAP pathway of lysine biosynthesis are lethal for cells exposed to lysine in the absence of DAP. In this paper, the substitution of the dapD gene of Escherichia coli with the kanamycin resistance gene from Tn903 is described and its possible uses are discussed.

引用

页码：49 / 51

页数：3

共 14 条

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