REDUCTION OF ERBB2 GENE-PRODUCT IN MAMMA CARCINOMA CELL-LINES BY ERBB2 MESSENGER-RNA-SPECIFIC AND TYROSINE KINASE CONSENSUS PHOSPHOROTHIOATE ANTISENSE OLIGONUCLEOTIDES

被引：49

作者：

BERTRAM, J ^{[1
]}

KILLIAN, M ^{[1
]}

BRYSCH, W ^{[1
]}

SCHLINGENSIEPEN, KH ^{[1
]}

KNEBA, M ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS CHEM,D-37075 GOTTINGEN,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1499

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A considerable reduction of up to 75% at the protein level of the erbB2 gene product was observed using phosphorothioate antisense oligonucleotides directed against specific sequences of the erbB2 mRNA. Antisense oligonucleotides used were the 14-mer (translation start region) and the 17-mer (3' translated region) all-phosphorothioate oligonucleotides (S-ODNs). Sense or random sequences were used as a control. The greatest reduction at the erbB2 protein level was obtained after a 24h incubation with a single dose of 2 mu M antisense S-ODN. The erbB2-mRNA-specific and the tyrosine kinase consensus antisense S-ODNs were comparably effective in inhibiting erbB2 expression. Addition of the transfection reagent DOTAP diminished the efficiency of erbB2 protein reduction by antisense S-ODNs after an incubation period of 24h but was more effective after 48h compared to the application of antisense S-ODNs alone. (C) 1994 Academic Press, Inc.

引用

页码：661 / 667

页数：7

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