COMPLEX-FORMATION IN YEAST DOUBLE-STRAND BREAK REPAIR - PARTICIPATION OF RAD51, RAD52, RAD55, AND RAD57 PROTEINS

The repair of DNA double-strand breaks in Saccharomyces cerevisiae requires genes of the RAD52 epistasis group, of which RAD55 and RAD57 are members, Here, we show that the x-ray sensitivity of rad55 and rad57 mutant strains is suppressible by overexpression of RAD51 or RAD52. Virtually complete suppression is provided by the simultaneous overexpression of RAD51 and RAD52. This suppression occurs at 23 degrees C, where these mutants are more sensitive to x-rays, as well as at 30 degrees C and 36 degrees C. In addition, a recombination defect of rad55 and rad57 mutants is similarly suppressed, Direct in vivo interactions between the Rad51 and Rad55 proteins, and between Rad55 and Rad57, have also been identified by using the two-hybrid system, These results indicate that these four proteins constitute part of a complex, a ''recombinosome,'' to effect the recombinational repair of double-strand breaks.