THERE ARE 2 MAJOR TYPES OF HEPATITIS-C VIRUS IN JAPAN

被引：317

作者：

ENOMOTO, N

TAKADA, A

NAKAO, T

DATE, T

机构：

[1] KANAZAWA MED UNIV,MED RES INST,DIV CANC RES,UCHINADA CHO,KAHOKU,ISHIKAWA 92002,JAPAN

[2] KANAZAWA MED UNIV,DEPT INTERNAL MED,DIV GASTROENTEROL,KAHOKU,ISHIKAWA 92002,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90494-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymerase chain reaction (PCR) was used to detect hepatitis C virus (HCV) in plasma from chronic non-A, non-B hepatitis patients. By choice of adequate primers, 19 of 24 samples (79%) were found positive. Sequence analysis of amplified 400 bp cDNA fragments encoding a portion of NS5 gene suggested that HCV can be classified into two types (named K1 and K2) in Japan. Slot blot hybridization of the fragments indicated that 13 were HCV-K1 and 6 were HCV-K2, which show 80% and 67% nucleotide sequence homology, respectively, with that of the prototype. © 1990.

引用

页码：1021 / 1025

页数：5

共 12 条

[1] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[2] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME
CHOO, QL
KUO, G
WEINER, AJ
OVERBY, LR
BRADLEY, DW
HOUGHTON, M
[J]. SCIENCE, 1989, 244 (4902) : 359 - 362
[3] GENERATION OF SINGLE-STRANDED-DNA BY THE POLYMERASE CHAIN-REACTION AND ITS APPLICATION TO DIRECT SEQUENCING OF THE HLA-DQA LOCUS
GYLLENSTEN, UB
ERLICH, HA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) : 7652 - 7656
[4] Houghton M, 1988, European patent application, Patent No. [88,310,922-5, 883109225]
[5] KANEKO S, 1990, LANCET, P976
[6] JAPANESE ISOLATES OF THE NON-A, NON-B HEPATITIS VIRAL GENOME SHOW SEQUENCE VARIATIONS FROM THE ORIGINAL ISOLATE IN THE USA
KATO, N
OHKOSHI, S
SHIMOTOHNO, K
[J]. PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 1989, 65 (09): : 219 - 223
[7] A CDNA FRAGMENT OF HEPATITIS-C VIRUS ISOLATED FROM AN IMPLICATED DONOR OF POST-TRANSFUSION NON-A, NON-B HEPATITIS IN JAPAN
KUBO, Y
TAKEUCHI, K
BOONMAR, S
KATAYAMA, T
CHOO, QL
KUO, G
WEINER, AJ
BRADLEY, DW
HOUGHTON, M
SAITO, I
MIYAMURA, T
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (24) : 10367 - 10372
[8] AN ASSAY FOR CIRCULATING ANTIBODIES TO A MAJOR ETIOLOGIC VIRUS OF HUMAN NON-A, NON-B-HEPATITIS
KUO, G
CHOO, QL
ALTER, HJ
GITNICK, GL
REDEKER, AG
PURCELL, RH
MIYAMURA, T
DIENSTAG, JL
ALTER, MJ
STEVENS, CE
TEGTMEIER, GE
BONINO, F
COLOMBO, M
LEE, WS
KUO, C
BERGER, K
SHUSTER, JR
OVERBY, LR
BRADLEY, DW
HOUGHTON, M
[J]. SCIENCE, 1989, 244 (4902) : 362 - 364
[9] LO WMD, 1988, NUCLEIC ACIDS RES, V17, P8719
[10] HEPATITIS-C VIRUS SHARES AMINO-ACID-SEQUENCE SIMILARITY WITH PESTIVIRUSES AND FLAVIVIRUSES AS WELL AS MEMBERS OF 2 PLANT-VIRUS SUPERGROUPS
MILLER, RH
PURCELL, RH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (06) : 2057 - 2061

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