A RAPID PURIFICATION METHOD FOR NEUROGRANIN, A BRAIN SPECIFIC CALMODULIN-BINDING PROTEIN-KINASE-C SUBSTRATE

被引:18
作者
DELOULME, JC [1 ]
SENSENBRENNER, M [1 ]
BAUDIER, J [1 ]
机构
[1] CNRS,CTR NEUROCHIM,NEUROBIOL ONTOGEN LAB,F-67084 STRASBOURG,FRANCE
关键词
PROTEIN KINASE-C SUBSTRATE; PURIFICATION; BRAIN (BOVINE);
D O I
10.1016/0014-5793(91)80473-G
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A rapid purification method is reported for bovine brain neurogranin, a calmodulin-binding protein kinase C (PKC) substrate. This method takes advantage of the fact that the protein remains soluble in 2.5% perchloric acid (PCA) and that it binds to a calmodulin-Sepharose column in the absence of calcium. Other PKC substrate proteins that remain to be identified were also found to share these two properties, suggesting that a class of calmodulin-binding PKC substrates may exist in the brain.
引用
收藏
页码:183 / 188
页数:6
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