INVITRO GROWTH-CHARACTERISTICS OF HUMAN ATHEROSCLEROTIC PLAQUE CELLS - COMPARISON OF CELLS FROM PRIMARY STENOSING AND RESTENOSING LESIONS OF PERIPHERAL AND CORONARY-ARTERIES

Cell size distribution and growth rates were studied in vitro in human plaque cells from advanced primary stenosing and fresh restenosing lesions of peripheral and coronary arteries. Cells were isolated either by the explant technique or by enzymatic disaggregation and were identified as smooth muscle cells by their typical growth pattern and their positive reaction with antibodies against smooth muscle α-actin. Endothelial cells were found in plaque specimens from coronary arteries but were only present in primary cultures. Smooth muscle cells from primary stenosing tissue (ps-SMC) exhibited a significantly lower growth rate in culture (0.15±0.04 population doublings per day; {Mathematical expression}) compared with cells from restenosing lesions (re-SMC; 0.60±0.13 population doublings per day; {Mathematical expression}). ps-SMC usually became senescent in their second passage, i.e., after 5-7 cumultive population doublings. re-SMC retained their high proliferative activity even after five passages (15 cumulative population doublings). Cell populations of both origins consisted of two distinct subpopulations which could be discriminated by cell size measurements: relatively small, predominant cells (cell diameter: 18.0±4 μm; {Mathematical expression}) and large fibroblast-like cells (cell diameter: 26.0±3 μm; {Mathematical expression}). The proportion of large cells was higher in cell populations derived from primary stenosing tissue. These results suggest that stenosing plaque tissue from human peripheral and coronary arteries consists of two smooth muscle cell subpopulations. The low proliferative activity of total smooth muscle cell populations of advanced primary stenosing lesions contrasts with the high mitotic activity of smooth muscle cells obtained from secondary stenosing intimal proliferates. © 1990 Springer-Verlag.