DETECTION OF BORRELIA-BURGDORFERI IN BIOLOGICAL SAMPLES USING THE POLYMERASE CHAIN-REACTION ASSAY

被引：35

作者：

DEBUE, M

GAUTIER, P

HACKEL, C

VANELSEN, A

HERZOG, A

BIGAIGNON, G

BOLLEN, A

机构：

[1] UNIV LIBRE BRUXELLES, SERV APPL GENET, 24 RUE IND, B-1400 NIVELLES, BELGIUM

[2] UNIV ESTADUAL CAMPINAS, FAC CIENCIAS MED, DEPT GENET MED, BR-13100 CAMPINAS, SP, BRAZIL

[3] CATHOLIC UNIV LOUVAIN, CLIN UNIV ST LUC, SERV MICROBIOL, B-1200 BRUSSELS, BELGIUM

来源：

RESEARCH IN MICROBIOLOGY | 1991年 / 142卷 / 05期

关键词：

BORRELIA-BURGDORFERI; LYME DISEASE; PCR; DIAGNOSIS; CEREBROSPINAL AND SYNOVIAL FLUIDS;

D O I：

10.1016/0923-2508(91)90189-H

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers, OspBpc3/pc4, outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.

引用

页码：565 / 572

页数：8

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