SCREENING ASSAY FOR DIOXIN-LIKE COMPOUNDS BASED ON COMPETITIVE-BINDING TO THE MURINE HEPATIC AH RECEPTOR .1. ASSAY DEVELOPMENT

被引：25

作者：

SCHNEIDER, UA ^{[1
]}

BROWN, MM ^{[1
]}

LOGAN, RA ^{[1
]}

MILLAR, LC ^{[1
]}

BUNCE, NJ ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT CHEM & BIOCHEM,GUELPH,ON N1G 2W1,CANADA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 1995年 / 29卷 / 10期

关键词：

D O I：

10.1021/es00010a021

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

PCDDs, PCDFs, and related analytes were assayed by binding the analytes competitively with fixed aliquots of radiolabeled 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and C57BL/6 mouse hepatic Ah receptor. Additive binding to the receptor was observed for a wide range of structural types of chlorinated aromatic compounds. The results of the assays were expressed as TCDD equivalents, i.e.,the concentration of unlabeled TCDD that would give the same assay as the mixture of analytes. The detection limit was approximately 100 pg of TCDD equivalent when the reference radioligand was [H-3] TCDD. The assay does not require prior knowledge of the identities of the components of the mixture and is intended for use as a prescreening tool rather than as a replacement for the conventional analysis by GC/MS.

引用

页码：2595 / 2602

页数：8

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