RAPID PROCEDURE TO DETERMINE THE DNA-BASE COMPOSITION FROM SMALL AMOUNTS OF GRAM-POSITIVE BACTERIA

被引：63

作者：

EZAKI, T

SAIDI, SM

LIU, SL

HASHIMOTO, Y

YAMAMOTO, H

YABUUCHI, E

机构：

[1] Department of Microbiology, Gifu University, School of Medicine, Gifu

来源：

FEMS MICROBIOLOGY LETTERS | 1990年 / 67卷 / 1-2期

关键词：

DNA base composition; Gram-positive bacterium; Rapid procedure;

D O I：

10.1016/0378-1097(90)90180-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram-positive bacteria is described. Cells of Gram-positive bacteria were lysed with achromopeptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram-positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h. © 1990.

引用

页码：127 / 130

页数：4

共 15 条

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