PURIFICATION OF PROTEINS ON AN EPOXY-ACTIVATED SUPPORT BY HIGH-PERFORMANCE AFFINITY-CHROMATOGRAPHY

被引：16

作者：

PHILLIPS, DJ

BELLALDEN, B

CAVA, M

GROVER, ER

MANDEVILLE, WH

MASTICO, R

SAWLIVICH, W

VELLA, G

WESTON, A

机构：

[1] UNIV LEEDS,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

[2] UNIV RHODE ISL,KINGSTON,RI 02881

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 536卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)89239-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of a rigid silica-based packing material with large particle and pore size, 37-55-mu-m and 500 angstrom pore, for affinity chromatography makes it possible to combine high selectivity with short analysis times. Both large and small molecules have been covalently bonded to the Protein-Pak(TM) Affinity Epoxy-Activated bulk packing for purification of glycoproteins, immunoglobulins, enzymes, lectins and other proteins. Recombinant protein A, GammaBind(TM) G, heparin, Cibacron Blue F3G-A, sulfanilamide, N-acetyl-D-glucosamine, concanavalin A and aminophenylboronic acid were covalently attached to the affinity packing for selective purification of proteins.

引用

页码：95 / 106

页数：12

共 11 条

[1] SELECTIVE ENZYME PURIFICATION BY AFFINITY CHROMATOGRAPHY [J].