A GENETIC MODEL FOR INTERACTION OF THE HOMEODOMAIN RECOGNITION HELIX WITH DNA

被引：200

作者：

HANES, SD ^{[1
]}

BRENT, R ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115

来源：

SCIENCE | 1991年 / 251卷 / 4992期

关键词：

D O I：

10.1126/science.1671176

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Bicoid homeodomain protein controls anterior development in the Drosophila embryo by binding to DNA and regulating gene expression. With the use of genetic assays in yeast, the interaction between the Bicoid homeodomain and a series of mutated DNA sites was studied. These experiments defined important features of homeodomain binding sites, identified specific amino acid-base pair contacts, and suggested a model for interaction of the recognition alpha-helices of Bicoid and Antennapedia-class homeodomain proteins with DNA. The model is in general agreement with results of crystallographic and magnetic resonance studies, but differs in important details. It is likely that genetic studies of protein-DNA interaction will continue to complement conventional structural approaches.

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页码：426 / 430

页数：5

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