A NEW APPROACH TO THE IMMUNOCYTOCHEMISTRY OF CAMP - INITIAL CHARACTERIZATION OF ANTIBODIES AGAINST ACROLEIN-FIXED CAMP

被引：4

作者：

DEVENTE, J ^{[1
]}

SCHIPPER, J ^{[1
]}

STEINBUSCH, HWM ^{[1
]}

机构：

[1] SOLVAY DUPHAR BV,DEPT PHARMACOL,1380 DA WEESP,NETHERLANDS

来源：

HISTOCHEMISTRY | 1993年 / 99卷 / 06期

关键词：

D O I：

10.1007/BF00274098

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A method is described to couple cyclic adenosine 3',5' monophosphate (cAMP) to a carrier protein by means of acrolein. Antibodies against this conjugate were raised in mice. These antibodies proved to be highly specific for acrolein-fixed cAMP in a gelatin model system. Slices (300 mum in thickness) from rat cerebral cortex were incubated in vitro and the dopaminergic control of adenylate cyclase activity was drug-manipulated. This manipulation was visualized by application of the cAMP-antisera on cryostat sections of the acrolein fixed slices.

引用

页码：457 / 462

页数：6

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