FLOW CYTOMETRIC STUDIES DURING CULTURE OF SACCHAROMYCES-CEREVISIAE

被引：36

作者：

SCHEPER, T

HOFFMANN, H

SCHUGERL, K

机构：

[1] Univ of Hannover, Hanover, West Ger, Univ of Hannover, Hanover, West Ger

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1987年 / 9卷 / 07期

关键词：

BIOLOGICAL MATERIALS - Cells - MICROBIOLOGY - Laser Applications;

D O I：

10.1016/0141-0229(87)90134-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Double-beam laser flow cytometry has been used to analyse samples from different Saccharomyces cerevisiae cultivation processes (batch and continuous culture). Using this technique, the biological parameters of cell size, and protein, RNA and DNA content can be determined. It is possible to study the budding behaviour from the cell size distribution as well as from DNA analysis. In oscillating continuous yeast cultures, flow cytometry studies were performed in addition to on-line NADH measurements. The effect of cell recycling on yeast metabolism was also the object of cytometric studies. Structured biological data can be obtained by this analytical technique, and the effect of different cultivation conditions on cell biology can be determined.

引用

页码：399 / 405

页数：7

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