HEAT-SHOCK RESPONSE OF RAT HEPATOMA VARIANT CELLS

The effect of mild heat shock on protein synthesis was examined in differentiated and dedifferentiated, glucocorticoid-sensitive and resistant clones of H4IIEC3 rat hepatoma cells by one- and two-dimensional gel electrophoresis of [S-35]methionine-labeled proteins. Among the major heat-shock proteins, five were induced in all hepatoma clones. Certain members of the HSP70 family and the corresponding mRNAs were only slightly inducible in the glucocorticoid-resistant variants, but were strongly inducible in the sensitive ones. Three other proteins lacked heat inducibility in the dedifferentiated clones. The constitutive level of one major heat-shock protein was elevated in all dedifferentiated variants. These results show that the stage of differentiation influences the expression of heat-shock genes of hepatoma cells. We found no correlation between the elevated constitutive or induced level of heat-shock proteins and heat resistance.